MntP and YiiP Contribute to Manganese Efflux in Salmonella enterica Serovar Typhimurium under Conditions of Manganese Overload and Nitrosative Stress

A Ouyang; KM Gasner; SL Neville; CA McDevitt; ER Frawley

Journal article

MntP and YiiP Contribute to Manganese Efflux in Salmonella enterica Serovar Typhimurium under Conditions of Manganese Overload and Nitrosative Stress

A Ouyang, KM Gasner, SL Neville, CA McDevitt, ER Frawley

Microbiology Spectrum | Published : 2022

DOI: 10.1128/spectrum.01316-21

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Abstract

The divalent transition metal cation manganese is important for protein function, particularly under conditions of iron limitation, nitrosative stress, and oxidative stress, but can mediate substantial toxicity in excess. Salmonella enterica serovar Typhimurium possesses multiple manganese importers, but the pathways for manganese efflux remain poorly defined. The S. Typhimurium ATCC 14028s genome was analyzed for putative manganese export pathways, which identified a previously uncharacterized homologue of the Escherichia coli manganese exporter mntP, stm1834, and two cation diffusion facilitator family transporters, zitB (stm0758) and yiiP (stm4061). Manganese acquisition by S. Typhimurium..

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University of Melbourne Researchers

Stephanie Neville Author

Christopher McDevitt Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

We thank Sarah Hasty for technical assistance with ICP-MS sample preparation and Joyce Karlinsey for helpful discussion and assistance with construction of the DyiiP mutant. A.O., K.M.G., and E.R.F. were supported by startup funding from Rhodes College. S.L.N. was supported by National Health and Medical Research Council (NHMRC) Early Career Research Fellowship 1142695. C.A.M. is supported by NHMRC grants 1140554 and 1180826 and an Australian Research Council Future Fellowship (FT170100006). The funders had no role in study design, data collection, interpretation, or the decision to submit the work for publication.